| Rang | Serie | Baan | Naam | Land | Tijd | Bijz. |
| 1 | 6 | 4 | King , Lilly Lilly King |  Verenigde Staten | 29.76 | Q |
| 2 | 5 | 4 | Jefimova , Joelia Joelia Jefimova |  Rusland | 29.99 | Q |
| 3 | 5 | 3 | Castiglioni , Arianna Arianna Castiglioni |  Italië | 30.33 | Q |
| 4 | 6 | 5 | Meili , Katie Katie Meili | Verenigde Staten | 30.37 | Q |
| 5 | 4 | 5 | Johansson , Jennie Jennie Johansson |  Zweden | 30.40 | Q |
| 6 | 4 | 4 | Meilutytė , Rūta Rūta Meilutytė |  Litouwen | 30.58 | Q |
| 7 | 5 | 6 | Hansen , Jessica Jessica Hansen |  Australië | 30.59 | Q |
| 8 | 5 | 5 | Vasey , Sarah Sarah Vasey | .svg.png) Verenigd Koninkrijk | 30.71 | Q |
| 9 | 4 | 2 | Ivanejeva , Natalja Natalja Ivanejeva | Rusland | 30.81 | Q |
| 10 | 6 | 6 | Laukkanen , Jenna Jenna Laukkanen |  Finland | 30.82 | Q |
| 11 | 4 | 6 | Lúthersdóttir , Hrafnhildur Hrafnhildur Lúthersdóttir |  IJsland | 30.88 | Q |
| 12 | 6 | 7 | Kivirinta , Veera Veera Kivirinta | Finland | 30.89 | Q |
| 13 | 4 | 3 | Carraro , Martina Martina Carraro | Italië | 30.92 | Q |
| 14 | 6 | 3 | Suzuki , Satomi Satomi Suzuki |  Japan | 30.95 | Q |
| 15 | 5 | 1 | Nicol , Rachel Rachel Nicol | .svg.png) Canada | 31.02 | Q |
| 16 | 4 | 1 | Suo Ran |  China | 31.22 | Q |
| 17 | 6 | 8 | Pedersen , Rikke Møller Rikke Møller Pedersen |  Denemarken | 31.29 | |
| 18 | 4 | 7 | Sztandera , Dominika Dominika Sztandera |  Polen | 31.31 | |
| 19 | 6 | 2 | Liver , Maria Maria Liver |  Oekraïne | 31.33 | |
| 20 | 5 | 2 | Hansson , Sophie Sophie Hansson | Zweden | 31.44 | |
| 21 | 5 | 7 | Ceballos , Macarena Macarena Ceballos |  Argentinië | 31.65 | |
| 22 | 6 | 1 | Chocová , Petra Petra Chocová |  Tsjechië | 31.69 | |
| 23 | 4 | 8 | Ivry , Amit Amit Ivry |  Israël | 31.70 | |
| 24 | 3 | 4 | Bjørnsen , Susann Susann Bjørnsen |  Noorwegen | 31.75 | |
| 25 | 6 | 0 | Corbett , Kaylene Kaylene Corbett |  Zuid-Afrika | 31.91 | |
| 26 | 5 | 0 | Romanjuk , Maria Maria Romanjuk |  Estland | 31.92 | |
| 27 | 4 | 0 | Sztankovics , Anna Anna Sztankovics |  Hongarije | 31.94 | |
| 28 | 5 | 8 | Podmaníková , Andrea Andrea Podmaníková |  Slowakije | 32.04 | |
| 29 | 4 | 9 | Toledo , Mercedes Mercedes Toledo |  Venezuela | 32.24 | |
| 30 | 6 | 9 | Kolidzeja , Dana Dana Kolidzeja |  Letland | 32.59 | |
| 31 | 5 | 9 | Evato , Anandia Anandia Evato |  Indonesië | 32.62 | |
| 32 | 3 | 6 | Paljk , Tilka Tilka Paljk |  Zambia | 32.72 | |
| 33 | 3 | 2 | Micallef , Amy Amy Micallef |  Malta | 33.46 | |
| 34 | 3 | 5 | Leter , Evita Evita Leter |  Suriname | 33.77 | |
| 35 | 3 | 3 | Talanova , Dariya Dariya Talanova |  Kirgizië | 33.85 | |
| 36 | 3 | 7 | Rivas , Vanessa Vanessa Rivas |  Dominicaanse Republiek | 34.40 | |
| 37 | 3 | 8 | Cherebin , Oreoluwa Oreoluwa Cherebin |  Grenada | 34.88 | |
| 38 | 3 | 1 | Mumladze , Meri Meri Mumladze |  Georgië | 34.91 | |
| 39 | 1 | 4 | Jang Myong-gyong |  Noord-Korea | 35.11 | |
| 40 | 2 | 1 | Grand'Pierre , Naomy Naomy Grand'Pierre |  Haïti | 35.85 | |
| 41 | 2 | 3 | Khatun , Mahfuza Mahfuza Khatun |  Bangladesh | 36.53 | |
| 42 | 2 | 4 | Furgeson , Colleen Colleen Furgeson |  Marshalleilanden | 36.97 | |
| 43 | 3 | 0 | Zhdrella , Melisa Melisa Zhdrella |  Kosovo | 37.06 | |
| 44 | 2 | 7 | Ouedraogo , Angelika Angelika Ouedraogo |  Burkina Faso | 37.89 | |
| 45 | 1 | 7 | Klimyk , Karina Karina Klimyk |  Tadzjikistan | 38.47 | |
| 46 | 2 | 2 | Yesayan , Elen Elen Yesayan |  Armenië | 38.67 | |
| 47 | 1 | 6 | Aishath , Sajina Sajina Aishath |  Malediven | 39.74 | |
| 48 | 2 | 0 | Budcharern , Siri Arun Siri Arun Budcharern |  Laos | 40.57 | |
| 49 | 2 | 6 | Zangwio , Laila Laila Zangwio |  Ghana | 42.03 | |
| 50 | 1 | 1 | Mahamane , Roukaya Roukaya Mahamane |  Niger | 46.49 | |
| 51 | 3 | 9 | Jalloh , Bunturabie Bunturabie Jalloh |  Sierra Leone | 49.38 | |
| 52 | 2 | 5 | Conde , Fatoumata Fatoumata Conde |  Guinee | 53.04 | |
| 1 | 5 | Konate , Fatoumata Fatoumata Konate |  Mali | DNS |
| 1 | 2 | Diagne , Fatou Fatou Diagne |  Senegal |
| 2 | 8 | Ahyee , Kokoe Kokoe Ahyee |  Ivoorkust |
| 2 | 9 | Rwabukamba , Albertine Albertine Rwabukamba |  Rwanda | DSQ |
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