| Rang | Serie | Baan | Naam | Land | Tijd | Bijz. |
| 1 | 3 | 4 | Hosszú , Katinka Katinka Hosszú |  Hongarije | 4:33.90 | Q |
| 2 | 3 | 5 | Belmonte , Mireia Mireia Belmonte |  Spanje | 4:35.29 | Q |
| 3 | 3 | 3 | Beisel , Elizabeth Elizabeth Beisel |  Verenigde Staten | 4:36.18 | Q |
| 4 | 2 | 6 | Pickrem , Sydney Sydney Pickrem | .svg.png) Canada | 4:36.25 | Q |
| 5 | 3 | 6 | Shimizu , Sakiko Sakiko Shimizu |  Japan | 4:36.43 | Q |
| 6 | 2 | 3 | Smith , Leah Leah Smith | Verenigde Staten | 4:36.94 | Q |
| 7 | 2 | 4 | Ohashi , Yui Yui Ohashi | Japan | 4:36.97 | Q |
| 8 | 2 | 5 | Miley , Hannah Hannah Miley | .svg.png) Verenigd Koninkrijk | 4:37.14 | Q |
| 9 | 3 | 2 | Kim Seo-yeong |  Zuid-Korea | 4:39.80 | |
| 10 | 3 | 7 | Nguyễn Thị Ánh Viên |  Vietnam | 4:40.39 | |
| 11 | 2 | 8 | Maranhão , Joanna Joanna Maranhão |  Brazilië | 4:41.29 | |
| 12 | 2 | 1 | Jakabos , Zsuzsanna Zsuzsanna Jakabos | Hongarije | 4:41.40 | |
| 13 | 3 | 1 | Zhou Min |  China | 4:42.26 | |
| 14 | 3 | 0 | Güneş , Viktoriya Zeynep Viktoriya Zeynep Güneş |  Turkije | 4:42.27 | |
| 15 | 2 | 9 | Ye Shiwen | China | 4:43.40 | |
| 16 | 1 | 3 | McKeown , Kaylee Kaylee McKeown |  Australië | 4:43.61 | |
| 17 | 2 | 0 | Kaminskaya , Victoria Victoria Kaminskaya |  Portugal | 4:44.96 | |
| 18 | 3 | 8 | Závadová , Barbora Barbora Závadová |  Tsjechië | 4:46.75 | |
| 19 | 2 | 2 | Harvey , Mary-Sophie Mary-Sophie Harvey | Canada | 4:46.88 | |
| 20 | 2 | 7 | Wood , Abbie Abbie Wood | Verenigd Koninkrijk | 4:47.30 | |
| 21 | 1 | 6 | González , Monika Monika González |  Mexico | 4:48.00 | |
| 22 | 3 | 9 | Crevar , Anja Anja Crevar |  Servië | 4:48.53 | |
| 23 | 1 | 5 | Gasson , Helena Helena Gasson |  Nieuw-Zeeland | 4:49.35 | |
| 24 | 1 | 4 | Bardach , Virginia Virginia Bardach |  Argentinië | 4:53.22 | |
| 25 | 1 | 2 | Lebl , Ilektra Ilektra Lebl |  Griekenland | 4:56.95 | |
| 26 | 1 | 7 | Permatahani , Azzahra Azzahra Permatahani |  Indonesië | 4:57.00 | |
| 27 | 1 | 1 | Nysted , Sara Sara Nysted |  Faeröer | 5:21.21 | |
| 1 | 8 | Ghosh , Sayani Sayani Ghosh |  India | DNS |
Rome
