| Rang | Serie | Baan | Naam | Land | Tijd | Bijz. |
| 1 | 6 | 5 | Koga , Junya Junya Koga |  Japan | 24.54 | Q |
| 2 | 5 | 4 | Lacourt , Camille Camille Lacourt |  Frankrijk | 24.58 | Q |
| 3 | 6 | 4 | Xu Jiayu |  China | 24.78 | Q |
| 3 | 7 | 5 | Grevers , Matt Matt Grevers |  Verenigde Staten | 24.78 | Q |
| 5 | 5 | 3 | Stravius , Jérémy Jérémy Stravius | Frankrijk | 24.80 | Q |
| 6 | 7 | 7 | Kopelev , Jonatan Jonatan Kopelev |  Israël | 24.86 | Q |
| 6 | 7 | 4 | Ress , Justin Justin Ress | Verenigde Staten | 24.86 | Q |
| 8 | 5 | 5 | Tarasevitsj , Grigori Grigori Tarasevitsj |  Rusland | 24.88 | Q |
| 9 | 5 | 6 | Bohus , Richárd Richárd Bohus |  Hongarije | 24.93 | Q |
| 10 | 6 | 6 | Sankovitsj , Pavel Pavel Sankovitsj |  Wit-Rusland | 24.94 | Q |
| 11 | 5 | 1 | Christou , Apostolos Apostolos Christou |  Griekenland | 24.99 | Q |
| 12 | 5 | 0 | Won Young-jun |  Zuid-Korea | 25.10 | Q |
| 12 | 7 | 3 | Polewka , Tomasz Tomasz Polewka |  Polen | 25.10 | Q |
| 14 | 6 | 3 | Guido , Guilherme Guilherme Guido |  Brazilië | 25.13 | Q |
| 15 | 4 | 3 | Acevedo , Javier Javier Acevedo | .svg.png) Canada | 25.16 | Q |
| 16 | 6 | 1 | I Gede Siman Sudartawa |  Indonesië | 25.17 | Q |
| 17 | 6 | 2 | Kolesnikov , Kliment Kliment Kolesnikov | Rusland | 25.23 | |
| 18 | 6 | 8 | Milli , Matteo Matteo Milli |  Italië | 25.24 | |
| 18 | 7 | 6 | Larkin , Mitch Mitch Larkin |  Australië | 25.24 | |
| 20 | 7 | 2 | Ulrich , Marek Marek Ulrich |  Duitsland | 25.27 | |
| 21 | 7 | 8 | Kasian , Bohdan Bohdan Kasian |  Oekraïne | 25.31 | |
| 22 | 5 | 8 | Beaver , Josh Josh Beaver | Australië | 25.44 | |
| 23 | 5 | 2 | Ryan , Shane Shane Ryan |  Ierland | 25.48 | |
| 24 | 6 | 7 | Barnea , Guy Guy Barnea | Israël | 25.52 | |
| 25 | 4 | 4 | Samy , Mohamed Mohamed Samy |  Egypte | 25.55 | |
| 25 | 7 | 1 | Staselovitsj , Viktar Viktar Staselovitsj | Wit-Rusland | 25.55 | |
| 27 | 4 | 6 | Hockin , Charles Charles Hockin |  Paraguay | 25.56 | |
| 27 | 5 | 7 | Balog , Gábor Gábor Balog | Hongarije | 25.56 | |
| 27 | 6 | 0 | Gurnytskyi , Dmytro Dmytro Gurnytskyi | Oekraïne | 25.56 | |
| 30 | 7 | 9 | Quah Zheng Wen |  Singapore | 25.58 | |
| 31 | 7 | 0 | Stokowski , Kacper Kacper Stokowski | Polen | 25.63 | |
| 32 | 6 | 9 | Lê Nguyễn Paul |  Vietnam | 25.64 | |
| 33 | 4 | 8 | Lie , Markus Markus Lie |  Noorwegen | 25.71 | |
| 34 | 4 | 5 | Tribuntsov , Ralf Ralf Tribuntsov |  Estland | 25.78 | |
| 35 | 5 | 9 | Stankevičius , Gytis Gytis Stankevičius |  Litouwen | 25.90 | |
| 36 | 4 | 2 | Molina , Robinson Robinson Molina |  Venezuela | 25.94 | |
| 37 | 4 | 7 | Hunter , Daniel Daniel Hunter |  Nieuw-Zeeland | 26.02 | |
| 38 | 4 | 9 | Lópes , Gabriel Gabriel Lópes |  Portugal | 26.05 | |
| 39 | 3 | 4 | Feldberg , Girts Girts Feldberg |  Letland | 26.27 | |
| 40 | 3 | 3 | Courtis , Christopher Christopher Courtis |  Barbados | 26.42 | |
| 41 | 3 | 5 | Benbara , Mehdi Mehdi Benbara |  Algerije | 26.57 | |
| 42 | 3 | 6 | Lahrichi , Driss Driss Lahrichi |  Marokko | 26.60 | |
| 43 | 3 | 7 | Prathapan , Madhu Madhu Prathapan |  India | 27.17 | |
| 44 | 3 | 2 | van der Colff , David David van der Colff |  Botswana | 27.67 | |
| 45 | 3 | 1 | Pornsiriporn , Narang Narang Pornsiriporn |  Thailand | 27.84 | |
| 46 | 2 | 7 | Ahmmed , Juwel Juwel Ahmmed |  Bangladesh | 28.50 | |
| 47 | 1 | 4 | Khan , Muhammad Muhammad Khan |  Pakistan | 28.97 | |
| 48 | 3 | 8 | Odlum-Smith , Jayhan Jayhan Odlum-Smith |  Saint Lucia | 29.95 | |
| 49 | 3 | 0 | Hatoum , Nabeel Nabeel Hatoum |  Palestina | 30.69 | |
| 50 | 1 | 3 | Thol Thoeun |  Kameroen | 30.72 | |
| 51 | 2 | 6 | Santisouk Inthavong |  Laos | 31.96 | |
| 52 | 2 | 1 | Irakose , Billy-Scott Billy-Scott Irakose |  Burundi | 33.14 | |
| 53 | 2 | 8 | Khorkashov , Ramziyor Ramziyor Khorkashov |  Tadzjikistan | 33.89 | |
| 54 | 2 | 5 | Al-Maleki , Ebrahim Ebrahim Al-Maleki |  Jemen | 34.07 | |
| 55 | 3 | 9 | Awoussou , Ablam Ablam Awoussou |  Brunei | 34.47 | |
| 1 | 5 | Ndir , Adama Adama Ndir |  Senegal | DNS |
| 2 | 4 | Beale , Moris Moris Beale |  Sierra Leone |
| 2 | 2 | Aonzoudine , Chaoili Chaoili Aonzoudine |  Comoren |
| 2 | 3 | Atta , Tano Tano Atta |  Ivoorkust |
| 4 | 0 | Pinzón , Omar Omar Pinzón |  Colombia |
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Liam Tancock
